Craniotomy in traumatic frontal sinus fracture cerebrospinal fluid leaks / 中国基层医药

Objective To assess the craniotomy in traumatic frontal sinus fracture cerebrospinal fluid( CSF) leaks.Methods Clinical data of 12 traumatic frontal sinus fracture CSF leaks from January 2010 to December 2014, who treated by craniotomy and conservation treatment was invalid were reviewed.Combined typical clinical presenta-tion and basicranial thin-layer computed tomography(CT),made qualitative diagnosis and localization.Craniotomy by bilateral coronary incision and epidural approach was performed.Repairation was mainly for the endocranium and the basicranium.Bone cement was used to reconstruct the osseous defect of the frontal sinus,and then with pedicle periosteal flap coverage.Dural defects was fixed with autogenous fascia.After operation,staying in bed and using anti-biotic for 7-14 days were required,while mannital or lumbar-drainage as needed.Results All 12 cases got posi-tive preoperative CT results.Craniotomy was performed,succeeded without reoperation.None of intracranial infection happened,while 1 case suffered from anosphrasia.Followed up for 3 -12 months, none CSF leaks relapsed. Conclusion Craniotomy by coronary incision,dispose the endocranium and the basicranium for the patients who suf-fered from frontal sinus fracture CSF leaks while conservation treatment is invalid,can obtain satisfied result.

Cloning and analysis of HSPC016 gene in dermal papilla cells / 解放军医学杂志

Objective To clone the full length cDNA sequence of HSPC016 gene, an aggregative growth related gene in dermal papilla cells (DPC). Analyze its characteristics and predict its biological function in the phase of growth and differentiation for DPC. Methods Rapid amplification of cDNA ends (RACE) technology was used for full length cDNA amplification. Bioinformtic methods were used to analyze the chromosome location, protein sequence, domain and possible biological function of the gene. Results Two isoforms of HSPC016 gene were obtained from DPC. They were 400bp and 493bp, respectively. The gene was mapped on chromosome 3 q21 31, and was conservative on evolution. HSPC016 protein had 64aa, belonged to PD053992 protein family; its functional domain was homologous to T2FA gene. Conclusion HSPC016 was a transcriptional modulatory gene. Its protein product may act as a subunit of a transcriptional complex and play a role on DPC growth and differentiation through modulating other genes' transcription within nucleus